Chinese translation for "insertion site"
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- 插入位点,克隆位点
Related Translations:
- Example Sentences:
| 1. | The insertion sites of tn5gusa5 were located by methods of tail - pcr and sequencing . for these 53 protease mutants , the insertion sites were distributed in 14 orfs
运用tail - por和测序的方法定位了转座子tn5gusa5的插入位置，经分析共插入14个orf中。 | | 2. | After amplifying genomic sequences flanked with tn5gusa5 by tail pcr and dna sequencing , then doing blast with genomic sequence of xcc 8004 , insertion sites of 10981 mutants were located precisely in xcc 8004 genome
经tail - pcr扩增tn5gusa5旁侧序列并测序，经与xcc8004基因组序列比对后获得精确定位的tn5gusa5插入突变体10981个。 | | 3. | The technique " improed coordinated timing of mechanical actiation of papillary muscle insertion sites and the remote decrease of functional mitral regurgitation secondary to left entricular reerse remodeling , " the authors write
作者指出，这一技术提高了乳头肌插入部机械出发和远隔部位二尖瓣重构导致的二尖瓣功能降低性返流之间的协同考虑。 | | 4. | Based on the blast analysis and other studies , osddl mutant was a multi - copy - insertion mutant , and one of the insertion sites was in an nptii like transposase gene , whereas osdd2 , a single - copy - insertion mutant was disrupted at a gene encoded wrky transcript factor
Osdd1突变体可能是多拷贝插入，其中一个插入到nptii转座酶类似基因。 osdd2突变体为单拷贝、插入到一个wrky类转录因子基因5翻译起始区附近。 | | 5. | The results showed that the f fragment , 728bp in length , could be a new gene with a little homology to the genes coding for polyketide synthetase or fatty - acid synthetase and the b fragment , about 4kb in length , is inferred to have repeat sequences around tn5 insertion site , in which there is homology to the wa 314 right arm of the high - pathogeniciry island of yersinia enterocolitica . to reveal any pathogenicity of enterobacter cloacae b8 and its mutated strains b8b and b8f to animals , the experiment with mice was carried out
结果显示， f片段长度为728bp ，与现有生物数据库的blast比较分析，发现该序列仅有局部短于1oobp的区域与polyketide合成酶基因或与脂肪酸合成酶基因有低的同源性，推测为一新基因； b片段长约4kb ，序列拼接结果推测靠近tn5插入位点部位有重复序列，对b片段tn5远端的部分序列进行blast比较，发现它与小肠结肠炎耶尔森氏菌的强毒力岛有一定的同源性。 | | 6. | And the two pah ' s of pcr primers that bind to the adapter and the sequence of f fragment close by tn5 respectively were also designed . the genomic dna of b8 was isolated , digested with bamh i , and ligated to the adapter . using the two pairs of the primers , two rounds of pcr were performed hi turn and a fragment of 239bp was amplified successfully . lt was proved by cloning and sequencing that 18bp of the fragment is the sequence opposite to f fragment on the left of tn5 insertion site in b8f , the other is part of the 728 bp of f fragment . this result makes it possible to continue to carry out chromosome walking , to clone and sequence the whole genes of b fragment and f fragment , and to reveal the antagonistic molecular mechanism of b8
试验研究设计并合成了由40和44个碱基的寡聚脱氧核苷酸组成的染色体爬行接头，在接头序列和测定的f片段近tn5的序列上，设计了2对染色体爬行用的pcr引物，从b8菌株中提取基因组dna ， bamhi酶切，与染色体爬行接头连接，依次用2对引物进行pcr ，扩增出239bp产物，经克隆、测序，发现其中18bp为扩增的相应于f片段在b8f菌株tn5插入位点对面的序列，其余则为f片段728bp序列的一部分，为进一步进行染色体爬行，克隆和测定整个b和f基因，揭示阳菌株的拮抗分子机制提供了技术资料贮备。 | | 7. | In addition , molecular experiments indicated that resistance was not relevant to copy number or to insertion sites , but instead to the repeated gfp structure and to methylation of genomic dna . pvx vectors were constructed that contained the s6 gene of rbsdv vi and the 14 kd fragment of bnyvv rna2
分子检测结果显示， gfp3植株中普遍存在gfp基因的沉默现象，且gfpdna的甲基化程度明显高于gfp1植株，说明这些植株对于病毒的抗性与目的基因的插入位点及拷贝数和无关，但与串联重复的gfp基因结构形式密切相关。 |
- Similar Words：
- "insertion rubber sheet" Chinese translation, "insertion sequence" Chinese translation, "insertion sequence selection" Chinese translation, "insertion sequences" Chinese translation, "insertion side effect" Chinese translation, "insertion slot" Chinese translation, "insertion sort" Chinese translation, "insertion sorting" Chinese translation, "insertion spanner" Chinese translation, "insertion strip" Chinese translation
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